Anti-inflammatory, Antioxidant and Cytotoxicity Potential of Methanolic Extract of Two Bangladeshi Bean Lablab Purpureus (l.) Sweet White and Purple

This study was subjected to investigate in vitro anti-inflammatory, antioxidant and cytotoxic properties of methanol extracts of two Bangladeshi bean pods namely Lablab purpureus L. sweet ‘white’ and ‘purple’. Protease Inhibition method was used to determine in-vitro anti-inflammatory properties. DPPH free radical scavenging method, total flavonoids, Total antioxidants and reducing power of the two samples were assessed for antioxidant properties and cytotoxicity potentials were assessed by the brine shrimp lethality test. In in-vitro anti-inflammatory investigation there is a linear relation of %inhibition for the white bean pods which indicates having positive anti-inflammatory property. In DPPH test the lowest and highest IC50 values are 430.00μg/ml and 853.13μg/ml, with L. purpureus sweet ‘purple’ and L. purpureus sweet ‘white‘ respectively and compared with standard ascorbic acid. In Cytotoxicity test LC50 value was found 960.06 μg/ml for L. purpureus (L.) sweet ‘purple’ and 66.5 μg/ml for L. purpureus sweet ‘white’, So L. purpureus sweet ‘white’ is more potent. The total Flavonoid contents of the test samples are 42.55±5.77 and 32.09±0.36 mg/g quercetin equivalents for ‘white’ and ‘purple’ respectively. L. purpureus (L.) Sweet White and Purple may posses’ potent anti-inflammatory, powerful antioxidant as well as good cytotoxic potential. INTRODUCTION: Phytochemicals are excessively found in plant kingdom . In medicine about 50% drug used are of plant origin, in developing countries nearly 80% people depends on traditional plant based medicines for primary health care ,and only a small fraction of medicinal plants has been tested . To solve the healthcare problems the attention to the medicinal plants are increasing world-wide. Plants are the origin of 25% of prescribed drugs in the world 3 and over 3000 species of plants have been reported to have anticancer properties . Antioxidants are added to a variety of foods to prevent or deter free radical induced lipid oxidation . If free radical production rate exceeds the normal capacity of the antioxidant defense mechanisms, substantial tissue injury results . Continuous exposure to chemicals and contaminants leads to increase the free radicals amount and causes irreversible oxidative damage which includes biological damage, DNA damage, diabetes, respiratory tract disorders, carcinogenesis and cellular degeneration related to aging 7, .